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rnp complex mixture  (Thermo Fisher)


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    Thermo Fisher rnp complex mixture
    Guide RNA design, RNA–protein complex formation <t>and</t> <t>electroporation.</t> A: A schematic representation of exon 2 of human MTTP gene. Three different guide RNAs (gRNAs) (red arrows) were designed within exon 2 for targeting. Forward and reverse primers (blue arrows) were used for amplification of the exon 2. B: Single guide RNA (sgRNA) consists of gRNA (red) and a recruiting sequence (green) for Cas9 enzyme. The <t>RNP</t> of sgRNA and Cas-9 protein complex was made in vitro by mixing 2.5 μmol of sgRNA and 2.0 μmol of Cas-9 protein in a microcentrifuge tube. This mixture was transfected into Huh-7 cells by electroporation using various electric pulses of different voltages (800 V, 1,250 V, 1,450 V, and 1,650 V) for 10 ms each. The control cells were also given similar pulses without RNP. The electroporated cells were resuspended in prewarmed media and plated in 6-well plate. C: A schematic representation showing the binding of gRNAs and expected cleavage sites on the target DNA. Cleavage followed by repair is expected to cause indels at cleavage site leading to gene disruption. CRISPR/Cas9, RNA-guided clustered regularly interspaced short palindromic repeats–associated sequence 9; RNP, ribonucleoprotein; sgRNA, single guide ribonucleic acid.
    Rnp Complex Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnp complex mixture/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    rnp complex mixture - by Bioz Stars, 2026-06
    86/100 stars

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    1) Product Images from "Generation of hepatoma cell lines deficient in microsomal triglyceride transfer protein"

    Article Title: Generation of hepatoma cell lines deficient in microsomal triglyceride transfer protein

    Journal: Journal of Lipid Research

    doi: 10.1016/j.jlr.2022.100257

    Guide RNA design, RNA–protein complex formation and electroporation. A: A schematic representation of exon 2 of human MTTP gene. Three different guide RNAs (gRNAs) (red arrows) were designed within exon 2 for targeting. Forward and reverse primers (blue arrows) were used for amplification of the exon 2. B: Single guide RNA (sgRNA) consists of gRNA (red) and a recruiting sequence (green) for Cas9 enzyme. The RNP of sgRNA and Cas-9 protein complex was made in vitro by mixing 2.5 μmol of sgRNA and 2.0 μmol of Cas-9 protein in a microcentrifuge tube. This mixture was transfected into Huh-7 cells by electroporation using various electric pulses of different voltages (800 V, 1,250 V, 1,450 V, and 1,650 V) for 10 ms each. The control cells were also given similar pulses without RNP. The electroporated cells were resuspended in prewarmed media and plated in 6-well plate. C: A schematic representation showing the binding of gRNAs and expected cleavage sites on the target DNA. Cleavage followed by repair is expected to cause indels at cleavage site leading to gene disruption. CRISPR/Cas9, RNA-guided clustered regularly interspaced short palindromic repeats–associated sequence 9; RNP, ribonucleoprotein; sgRNA, single guide ribonucleic acid.
    Figure Legend Snippet: Guide RNA design, RNA–protein complex formation and electroporation. A: A schematic representation of exon 2 of human MTTP gene. Three different guide RNAs (gRNAs) (red arrows) were designed within exon 2 for targeting. Forward and reverse primers (blue arrows) were used for amplification of the exon 2. B: Single guide RNA (sgRNA) consists of gRNA (red) and a recruiting sequence (green) for Cas9 enzyme. The RNP of sgRNA and Cas-9 protein complex was made in vitro by mixing 2.5 μmol of sgRNA and 2.0 μmol of Cas-9 protein in a microcentrifuge tube. This mixture was transfected into Huh-7 cells by electroporation using various electric pulses of different voltages (800 V, 1,250 V, 1,450 V, and 1,650 V) for 10 ms each. The control cells were also given similar pulses without RNP. The electroporated cells were resuspended in prewarmed media and plated in 6-well plate. C: A schematic representation showing the binding of gRNAs and expected cleavage sites on the target DNA. Cleavage followed by repair is expected to cause indels at cleavage site leading to gene disruption. CRISPR/Cas9, RNA-guided clustered regularly interspaced short palindromic repeats–associated sequence 9; RNP, ribonucleoprotein; sgRNA, single guide ribonucleic acid.

    Techniques Used: Electroporation, Amplification, Sequencing, In Vitro, Transfection, Binding Assay, CRISPR



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    rnp complex mixture  (Thermo Fisher)
    86
    Thermo Fisher rnp complex mixture
    Guide RNA design, RNA–protein complex formation <t>and</t> <t>electroporation.</t> A: A schematic representation of exon 2 of human MTTP gene. Three different guide RNAs (gRNAs) (red arrows) were designed within exon 2 for targeting. Forward and reverse primers (blue arrows) were used for amplification of the exon 2. B: Single guide RNA (sgRNA) consists of gRNA (red) and a recruiting sequence (green) for Cas9 enzyme. The <t>RNP</t> of sgRNA and Cas-9 protein complex was made in vitro by mixing 2.5 μmol of sgRNA and 2.0 μmol of Cas-9 protein in a microcentrifuge tube. This mixture was transfected into Huh-7 cells by electroporation using various electric pulses of different voltages (800 V, 1,250 V, 1,450 V, and 1,650 V) for 10 ms each. The control cells were also given similar pulses without RNP. The electroporated cells were resuspended in prewarmed media and plated in 6-well plate. C: A schematic representation showing the binding of gRNAs and expected cleavage sites on the target DNA. Cleavage followed by repair is expected to cause indels at cleavage site leading to gene disruption. CRISPR/Cas9, RNA-guided clustered regularly interspaced short palindromic repeats–associated sequence 9; RNP, ribonucleoprotein; sgRNA, single guide ribonucleic acid.
    Rnp Complex Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnp complex mixture/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    rnp complex mixture - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Guide RNA design, RNA–protein complex formation and electroporation. A: A schematic representation of exon 2 of human MTTP gene. Three different guide RNAs (gRNAs) (red arrows) were designed within exon 2 for targeting. Forward and reverse primers (blue arrows) were used for amplification of the exon 2. B: Single guide RNA (sgRNA) consists of gRNA (red) and a recruiting sequence (green) for Cas9 enzyme. The RNP of sgRNA and Cas-9 protein complex was made in vitro by mixing 2.5 μmol of sgRNA and 2.0 μmol of Cas-9 protein in a microcentrifuge tube. This mixture was transfected into Huh-7 cells by electroporation using various electric pulses of different voltages (800 V, 1,250 V, 1,450 V, and 1,650 V) for 10 ms each. The control cells were also given similar pulses without RNP. The electroporated cells were resuspended in prewarmed media and plated in 6-well plate. C: A schematic representation showing the binding of gRNAs and expected cleavage sites on the target DNA. Cleavage followed by repair is expected to cause indels at cleavage site leading to gene disruption. CRISPR/Cas9, RNA-guided clustered regularly interspaced short palindromic repeats–associated sequence 9; RNP, ribonucleoprotein; sgRNA, single guide ribonucleic acid.

    Journal: Journal of Lipid Research

    Article Title: Generation of hepatoma cell lines deficient in microsomal triglyceride transfer protein

    doi: 10.1016/j.jlr.2022.100257

    Figure Lengend Snippet: Guide RNA design, RNA–protein complex formation and electroporation. A: A schematic representation of exon 2 of human MTTP gene. Three different guide RNAs (gRNAs) (red arrows) were designed within exon 2 for targeting. Forward and reverse primers (blue arrows) were used for amplification of the exon 2. B: Single guide RNA (sgRNA) consists of gRNA (red) and a recruiting sequence (green) for Cas9 enzyme. The RNP of sgRNA and Cas-9 protein complex was made in vitro by mixing 2.5 μmol of sgRNA and 2.0 μmol of Cas-9 protein in a microcentrifuge tube. This mixture was transfected into Huh-7 cells by electroporation using various electric pulses of different voltages (800 V, 1,250 V, 1,450 V, and 1,650 V) for 10 ms each. The control cells were also given similar pulses without RNP. The electroporated cells were resuspended in prewarmed media and plated in 6-well plate. C: A schematic representation showing the binding of gRNAs and expected cleavage sites on the target DNA. Cleavage followed by repair is expected to cause indels at cleavage site leading to gene disruption. CRISPR/Cas9, RNA-guided clustered regularly interspaced short palindromic repeats–associated sequence 9; RNP, ribonucleoprotein; sgRNA, single guide ribonucleic acid.

    Article Snippet: For electroporation, 10 μl of Huh-7 cells and RNP complex mixture were aspirated into gold plated tips using pipette supplied with kit (# MPK1096, ThermoFisher Scientific).

    Techniques: Electroporation, Amplification, Sequencing, In Vitro, Transfection, Binding Assay, CRISPR